Unknown

Dataset Information

0

RNA-seq of mouse Lgr5+ intestinal stem cells and progenitors following Lkb1 deletion against controls


ABSTRACT: Perturbed intestinal epithelial homeostasis demonstrated as decreased Lgr5+ intestinal stem cells (Lgr5 ISCs) and increased secretory lineages were observed in our study where Lkb1 was specfically deleted in Lgr5 ISCs using Lgr5-EGFP-creERT2 (Tamoxifen) deletor. To gain mechanistic insight how Lkb1 maintains intestinal epithelial stem cell homeostasis, Lkb1 deficient ISCs (Lgr5-high cells) and progenitors (Lgr5-low cells) are isolated by flow cytometry and profiled by RNA sequencing to compare with controls (Lkb1 wild type ISCs and progenitors).

SUBMITTER: Sushil Tripathi 

PROVIDER: E-MTAB-6501 | BioStudies | 2020-02-29

SECONDARY ACCESSION(S): ERP107471

REPOSITORIES: biostudies

Similar Datasets

2020-02-29 | E-MTAB-6501 | ArrayExpress
2017-01-01 | S-EPMC5505276 | BioStudies
2017-01-01 | S-EPMC5376969 | BioStudies
2017-01-01 | S-EPMC5405134 | BioStudies
1000-01-01 | S-EPMC5732700 | BioStudies
2017-01-01 | S-EPMC5774992 | BioStudies
2019-01-01 | S-EPMC6485179 | BioStudies
2016-01-01 | S-EPMC5001892 | BioStudies
2016-01-01 | S-EPMC4961601 | BioStudies
1000-01-01 | S-EPMC3258636 | BioStudies