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Transcription profiling of Populus × canescens roots and leaves in reaction to fungal (Laccaria bicolor) volatiles

ABSTRACT: Populus × canescens plantlets were grown in Petri dishes on MS medium. 14 days old plantlets were co-incubated with L. bicolor without physical contact. After 2 and 7 days of co-incubation, roots and leaves were harvested.

ORGANISM(S): Populus tremula x Populus alba

PROVIDER: E-MTAB-8683 | BioStudies |

REPOSITORIES: biostudies

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Transcription profiling of Populus × canescens roots and leaves in reaction to fungal (Laccaria bicolor) volatiles

Project description:Populus × canescens plantlets were grown in Petri dishes on MS medium. 14 days old plantlets were co-incubated with L. bicolor without physical contact. After 2 and 7 days of co-incubation, roots and leaves were harvested.

2020-12-10 | E-MTAB-8683 | ArrayExpress

Transcription profiling of Arabidopsis thaliana root parts in reaction to fungal (Laccaria bicolor) volatiles

Project description:Arabidopsis thaliana plants were grown from seeds in Petri dishes on MS medium. 4 days old plants were co-incubated with L. bicolor without physical contact. After 2 days of co-incubation, roots were either harvested whole or separated into the root tip (ca. 1/4 of the whole root) and the lateral root zone (= remaining root).

| E-MTAB-8682 | BioStudies

Transcription profiling of Arabidopsis thaliana roots and leaves in reaction to fungal (Laccaria bicolor) volatiles

Project description:Arabidopsis thaliana plants were grown from seeds in Petri dishes on MS medium. 4 days old plants were co-incubated with L. bicolor without physical contact. Roots were harvested after 0, 2, 4 and 7 days of co-incubation, rosette leaves were harvested after 4 days of co-incubation.

| E-MTAB-8684 | BioStudies

Transcription profiling of Populus trichocarpa roots in response to species diversity levels

Project description:Changes in gene expression in P. trichocarpa leaves was measured in response to growth competition by P. x canescens.Square petri dishes (12 x 12 cm) were filled autoclaved sand, 3 plantlets and Woody Plant Medium (WPM). In each petri dish we are growing 3 plantlets. As control 3 plantlets of P. trichocarpa and for the experiment a mixture of P. canescens and P. trichocarpa were P. trichocarpa is all the time the middle plantlet.

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Transcription profiling of Populus trichocarpa leaves in response to species diversity levels

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Transcription profiling of Populus x canescens leaves to evaluate the effect of co-cultivation with ectomycorrhizal fungus Laccaria bicolor

Project description:Populus x canescens plants were co-cultivated with or without (control) the ectomycorrhizal fungus Laccaria bicolor. Leaves were harvested after 13 weeks of greenhouse cultivation.

| E-MTAB-8929 | BioStudies

Transcription profiling of Arabidopsis thaliana roots and leaves in reaction to fungal (Laccaria bicolor) volatiles

Project description:Arabidopsis thaliana plants were grown from seeds in Petri dishes on MS medium. 4 days old plants were co-incubated with L. bicolor without physical contact. Roots were harvested after 0, 2, 4 and 7 days of co-incubation, rosette leaves were harvested after 4 days of co-incubation.

2020-12-10 | E-MTAB-8684 | ArrayExpress

Transcription profiling of Populus x canescens leaves to evaluate the effect of co-cultivation with ectomycorrhizal fungus Laccaria bicolor

2021-03-01 | E-MTAB-8929 | ArrayExpress

Transcription profiling of Arabidopsis thaliana root parts in reaction to fungal (Laccaria bicolor) volatiles

2020-12-10 | E-MTAB-8682 | ArrayExpress

Transcriptome analyses of the Laccaria bicolor-Populus trichocarpa mycorrhiza development

Project description:Illumina GAIIx technology was used to generate mRNA profiles from the ectomycorrhizal fungi Laccaria bicolor colonizing roots of Populus trichocarpa. Samples were taken after two, four and 12 weeks of contact in order to identify mycorrhiza-regulated transcripts. 37bp reads were generated and aligned to the Populus trichocarpa (http://www.phytozome.net/poplar.php) and the Laccaria bicolor (http://genome.jgi-psf.org/Lacbi2/Lacbi2.home.html) reference genomes using CLC Genomics Workbench 6. mRNA profiles from Populus trichocarpa roots colonized by Laccaria bicolor for two, four and 12 weeks as well as from control roots and free-living mycelium were generated by using one lane of 37 bp Illumina GAIIx sequencing per sample.

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