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Transcription profiling of S. cervisiae wild type (BY4741) and Puf3 mutant cells grown in minimal medium supplemented with 3% glycerol


ABSTRACT: Three independent experiments: S. cervisiae wild-type (BY4741) and Puf3 mutant cells were grown in minimal medium supplemented with 3% glycerol. Cells were harvested in mid-log phase by centrifugation and total RNA was prepared by hot-phenol extraction. cDNA was prepared with oligo-dT and using a mixture of amino-allyl dUTP and dNTPs, fluorescently labeled (Cy3= wild-type, Cy5 = mutant) and hybridize on S. cerevisiae DNA microarray.

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-2623 | BioStudies | 2005-11-01

REPOSITORIES: biostudies

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