Dataset Information


Identification of the haem-binding subunit of cytochrome b-245.

ABSTRACT: Cytochrome b-245 from neutrophil plasma membranes contains two types of subunit with apparent molecular masses from gel electrophoresis in the presence of SDS of 23 kDa and 76-92 kDa. Radiation-inactivation analysis revealed a single-exponential decay process for the visible absorption of the haem chromophore in the membrane, corresponding to a molecular mass of 21 +/- 5 kDa for the haem-containing polypeptide chain. Sedimentation equilibrium of the cytochrome solubilized by the detergent Triton N101 showed that the protein was polydisperse, with a molecular mass of approx. 350 kDa for the smallest detectable species. In another detergent, n-octyl beta-O-glucopyranoside (octyl glucoside), the molecular mass of the haem-containing particle was found to be 20-30 kDa. Thus the quaternary structure of the protein breaks down in this detergent. The haem group is inferred to be attached to the smaller subunit.

PROVIDER: S-EPMC1133673 | BioStudies | 1989-01-01

REPOSITORIES: biostudies

Similar Datasets

1984-01-01 | S-EPMC1153509 | BioStudies
2001-01-01 | S-EPMC1222130 | BioStudies
2000-01-01 | S-EPMC1220756 | BioStudies
| S-EPMC1462683 | BioStudies
1992-01-01 | S-EPMC1130759 | BioStudies
1992-01-01 | S-EPMC1132840 | BioStudies
1992-01-01 | S-EPMC1132607 | BioStudies
| S-EPMC2243080 | BioStudies
1995-01-01 | S-EPMC1135831 | BioStudies
1991-01-01 | S-EPMC1151524 | BioStudies