Unknown

Dataset Information

0

Properties of cadmium-binding proteins in rat testes. Characteristics unlike metallothionein.


ABSTRACT: Since the exposure of rats to cadmium causes zinc to accumulate in metallothionein in liver and kidney but not in a similar protein in the testes, the properties of the low-Mr cadmium-binding proteins were investigated in rat testes. Weanling rats that had been given dietary cadmium for 6 weeks were injected with 109CdCl2 and subsequently killed, and the 109Cd-labelled low-Mr proteins from testes were purified. The pooled low-Mr cadmium-containing fractions from the gel-filtration (Sephadex G-75) columns were eluted through DEAE-Sephacel columns, yielding two peaks. Each of the individual peaks from this Sephacel column was further purified by rechromatography on DEAE-Sephacel and on Bio-Gel P-10 columns. Amino acid analysis of the two purified proteins revealed a low cysteine (about 3%) content, with aspartate, glutamate and glycine as the predominant amino acids. Thus these low-Mr cadmium-binding proteins induced by cadmium in rat testes do not appear to be metallothionein.

SUBMITTER: Deagen JT 

PROVIDER: S-EPMC1152742 | BioStudies | 1985-01-01

REPOSITORIES: biostudies

Similar Datasets

1986-01-01 | S-EPMC1147222 | BioStudies
1985-01-01 | S-EPMC1152851 | BioStudies
1984-01-01 | S-EPMC1153693 | BioStudies
1988-01-01 | S-EPMC1149039 | BioStudies
1986-01-01 | S-EPMC1152987 | BioStudies
1988-01-01 | S-EPMC1149010 | BioStudies
1984-01-01 | S-EPMC1153700 | BioStudies
1987-01-01 | S-EPMC1148630 | BioStudies
1984-01-01 | S-EPMC1153701 | BioStudies
1992-01-01 | S-EPMC1130910 | BioStudies