Unknown

Dataset Information

0

The carbohydrate components of arterial basement-membrane-like material. Studies on rabbit aortic myomedial cells in culture.


ABSTRACT: The carbohydrate composition of arterial basement-membrane-like material was investigated. Basement-membrane-like material was isolated from cultures of aortic myomedial cells by a sonication/differential-centrifugation technique. Purified basement-membrane-like material contained a total of 5% sugars, comprising glucose, galactose, mannose, fucose, sialic acid, glucosamine and galactosamine in the approximate molar proportions 3.2:3.5:3.4:3.2:1:5.5:3.1. In addition, small amounts of xylose were found. Analyses for uronic acid showed that glycosaminoglycans comprised about 1% of isolated basement-membrane-like material. The carbohydrate composition indicated the presence of complex-type oligosaccharides in addition to hydroxylysine-linked disaccharides. [3H]Glucosamine-labelled glycopeptides obtained by proteinase digestion and gel filtration were resistant to endo-beta-N-acetylglucosaminidase D, but more than 10% were susceptible to alpha-mannosidase, demonstrating the presence of high-mannose-type oligosaccharides. The distribution of carbohydrates among peptides of basement-membrane-like material on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis was investigated after labelling with [3H]mannose, [3H]fucose, [3H]galactose and [3H]glucosamine. Among peptides that appeared to carry carbohydrates were a proteoglycan(s) and seven glycoproteins in the molecular-weight range 120 000-700 000.

PROVIDER: S-EPMC1154421 | BioStudies | 1983-01-01

REPOSITORIES: biostudies

Similar Datasets

1983-01-01 | S-EPMC1154143 | BioStudies
1980-01-01 | S-EPMC1162317 | BioStudies
1991-01-01 | S-EPMC1149933 | BioStudies
1999-01-01 | S-EPMC1220104 | BioStudies
1981-01-01 | S-EPMC1162864 | BioStudies
1983-01-01 | S-EPMC1152130 | BioStudies
1976-01-01 | S-EPMC1172542 | BioStudies
1971-01-01 | S-EPMC1178045 | BioStudies
| S-EPMC3072268 | BioStudies
1980-01-01 | S-EPMC1162065 | BioStudies