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Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield.


ABSTRACT: Cathepsin D was purified by two-step affinity chromatography on concanavalin A-- and pepstatin--Sepharose. The main purification was achieved by washing the enzyme bound to the pepstatin--Sepharose column with buffered 6 M-urea. This step separated cathepsin D from all low- and high-molecular-weight impurities. Although the 1700-fold purified acid proteinase was homogeneous on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, it still showed microheterogeneity.

SUBMITTER: Afting EG 

PROVIDER: S-EPMC1163155 | BioStudies | 1981-01-01

SECONDARY ACCESSION(S): 10.1042/bj1970519

REPOSITORIES: biostudies

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