Unknown

Dataset Information

0

Studies on the cholesterol ester hydrolase of Trogoderma (Coleoptera).


ABSTRACT: The enzyme cholesterol ester hydrolase (EC 3.1.1.13) was detected in the larvae of the khapra beetle, Trogoderma granarium (Everts). The pH and temperature optima for the enzyme were 6.6 degrees and 37 degrees C respectively. The mol.wt. of the enzyme was 76000-80000. The enzyme was equally effective in hydrolysing cholesteryl acetate, stearate and oleate. Cholesterol derivatives, namely the chloride and the methyl ether, inhibited the enzyme activity almost completely. It was also inhibited completely by p-hydroxymercuribenzoate. This inhibition was reversed by the addition of dithiothreitol, reduced glutathione or cysteine. The enzyme activity was associated predominantly with the 104000 g fraction.

SUBMITTER: Agarwal HC 

PROVIDER: S-EPMC1163822 | BioStudies | 1976-01-01

SECONDARY ACCESSION(S): 10.1042/bj1570111

REPOSITORIES: biostudies

Similar Datasets

| PRJNA489597 | ENA
1974-01-01 | S-EPMC1168427 | BioStudies
2017-01-01 | S-EPMC5522931 | BioStudies
1969-01-01 | S-EPMC1187728 | BioStudies
2019-01-01 | S-EPMC6375610 | BioStudies
1975-01-01 | S-EPMC1172470 | BioStudies
1967-01-01 | S-EPMC1270263 | BioStudies
1965-01-01 | S-EPMC1214347 | BioStudies
2017-01-01 | S-EPMC5681968 | BioStudies
1977-01-01 | S-EPMC1164932 | BioStudies