Unknown

Dataset Information

0

Saccharomyces cerevisiae as a model system to define the chromosomal instability phenotype.


ABSTRACT: Translocations, deletions, and chromosome fusions are frequent events seen in cancers with genome instability. Here we analyzed 358 genome rearrangements generated in Saccharomyces cerevisiae selected by the loss of the nonessential terminal segment of chromosome V. The rearrangements appeared to be generated by both nonhomologous end joining and homologous recombination and targeted all chromosomes. Fifteen percent of the rearrangements occurred independently more than once. High levels of specific classes of rearrangements were isolated from strains with specific mutations: translocations to Ty elements were increased in telomerase-defective mutants, potential dicentric translocations and dicentric isochromosomes were associated with cell cycle checkpoint defects, chromosome fusions were frequent in strains with both telomerase and cell cycle checkpoint defects, and translocations to homolog genes were seen in strains with defects allowing homoeologous recombination. An analysis of human cancer-associated rearrangements revealed parallels to the effects that strain genotypes have on classes of rearrangement in S. cerevisiae.

SUBMITTER: Putnam CD 

PROVIDER: S-EPMC1190249 | BioStudies | 2005-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2009-01-01 | S-EPMC2712687 | BioStudies
2016-01-01 | S-EPMC5065131 | BioStudies
2007-01-01 | S-EPMC1899885 | BioStudies
2010-01-01 | S-EPMC2918500 | BioStudies
2008-01-01 | S-EPMC2585166 | BioStudies
2009-09-03 | GSE17903 | GEO
2011-01-01 | S-EPMC3428953 | BioStudies
2010-01-01 | S-EPMC2818350 | BioStudies
1000-01-01 | S-EPMC3597658 | BioStudies
2014-01-01 | S-EPMC3907307 | BioStudies