Dataset Information


Interaction between Ustilago maydis REC2 and RAD51 genes in DNA repair and mitotic recombination.

ABSTRACT: A gene encoding a Ustilago maydis Rad51 orthologue has been isolated, rad51-1, a mutant constructed by disrupting the gene, was as sensitive to killing by ultraviolet light and gamma radiation as the rec2-1 mutant and slightly more sensitive to killing by methyl methanesulfonate. There was no suppression of killing by ultraviolet light when a rec2-1 strain was transformed with a multicopy plasmid containing RAD51, nor was there suppression when rad51-1 was transformed with a multicopy plasmid containing REC2. Recombination proficiency as measured by a gap repair assay was diminished in both rec2-1 and rad51-1 strains. In rec2-1 the frequency of recombination was decreased, but the spectrum of events was similar to that observed in wild type, while in rad51-1 the frequency as well as the spectrum of recombination events were different. Studies with the rec2-1 rad51-1 double mutant indicated that there was epistasis in the action of REC2 and RAD51 in certain repair and recombination functions, but some measure of independent action in other functions.

PROVIDER: S-EPMC1207791 | BioStudies | 1997-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2000-01-01 | S-EPMC1461025 | BioStudies
| S-EPMC133769 | BioStudies
| S-EPMC6795472 | BioStudies
1994-01-01 | S-EPMC358822 | BioStudies
1994-01-01 | S-EPMC196726 | BioStudies
| S-EPMC5499189 | BioStudies
| S-EPMC2876378 | BioStudies
1995-01-01 | S-EPMC231949 | BioStudies
| S-EPMC1952148 | BioStudies
| S-EPMC6707447 | BioStudies