Dataset Information


Contribution of copper binding to the inhibition of lipid oxidation by plasmalogen phospholipids.

ABSTRACT: The role of plasmalogen phospholipids for copper-induced lipid oxidation was evaluated. Using 1H-NMR we observed that the copper (CuSO4)-promoted oxidative degradation of polyunsaturated fatty acids in micellar solution was dose-dependently attenuated by the plasmalogen lysoplasmenylethanolamine from bovine brain (lysoBP-PtdEtn). This was due to a direct interaction of copper ions with the plasmalogen-specific enol ether double bond. The enol ether methine 1H signal decreased on the addition of copper, saturation being reached at a molar ratio of lysoBP-PtdEtn to copper of 1:1. The original 1H signal was recovered almost completely after the addition of EDTA. Enrichment of micelles and low-density lipoproteins (LDLs) with plasmalogen phospholipids led to a decrease in the Cu(II) concentration in the aqueous media. After loading of LDLs in vitro with BP-PtdEtn, the LDL-dependent formation of Cu(I) was decreased, in particular in particles experimentally supplemented with alpha-tocopherol. The suppression of copper-promoted lipid oxidation that was observed in the presence of plasmalogen phospholipids plus alpha-tocopherol was greater than the sum of the protective effects elicited by the two substances alone. In conclusion, the formation of a complex between copper ions and the plasmalogens accounts partly for their inhibition of copper-induced lipid oxidation.

PROVIDER: S-EPMC1220260 | BioStudies | 1999-01-01

REPOSITORIES: biostudies

Similar Datasets

1997-01-01 | S-EPMC1218386 | BioStudies
1999-01-01 | S-EPMC1220115 | BioStudies
| S-EPMC2807911 | BioStudies
| S-EPMC5341075 | BioStudies
| S-EPMC8671113 | BioStudies
1990-01-01 | S-EPMC1136900 | BioStudies
1965-01-01 | S-EPMC1206435 | BioStudies
| S-EPMC3033967 | BioStudies
| S-EPMC8048887 | BioStudies
1965-01-01 | S-EPMC1215201 | BioStudies