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The use of fluorine-containing carboxylic acids for the large-scale isolation of highly polymerized deoxyribonucleic acids.


ABSTRACT: 1. The precipitation of the proteins of calf-thymus nucleohistone and herringsperm nucleoprotamine with the salts of fluorine-containing carboxylic acids has been studied. Pentadecafluoro-octanoate gave a precipitate that could be sedimented almost completely by centrifuging at 2350g for 20min. omega-H-Hexadecafluorononanoate, omega-H-eicosafluoro-undecanoate and dodecyl sulphate gave precipitates that could only be partly sedimented under these conditions. 2. A method has been developed for the isolation of highly polymerized DNA on a large scale from calf thymus and herring sperm by the use of pentadecafluorooctanoate. This is more convenient and less tedious than existing methods.

SUBMITTER: Jones AS 

PROVIDER: S-EPMC1264955 | BioStudies | 1966-01-01

SECONDARY ACCESSION(S): 10.1042/bj0990049

REPOSITORIES: biostudies

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