Dataset Information


Human herpesvirus 8 glycoprotein B (gB), gH, and gL can mediate cell fusion.

ABSTRACT: Herpesvirus entry into cells and herpesvirus-induced cell fusion are related processes in that virus penetration proceeds by fusion of the viral envelope and cell membrane. To characterize the human herpesvirus 8 (HHV-8) glycoproteins that can mediate cell fusion, a luciferase reporter gene activation assay was used. Chinese hamster ovary (CHO) cells expressing the HHV-8 glycoproteins of interest along with a luciferase reporter gene under the control of the T7 promoter were cocultivated with human cells transfected with T7 RNA polymerase. Because HHV-8 glycoprotein B (gB) expressed in CHO cells localizes to the perinuclear region, a truncated form of gB (designated gB(MUT)) that lacks putative endocytosis signals was constructed by deletion of the distal 58 amino acids of the cytoplasmic tail. HHV-8 gB(MUT) was expressed efficiently on the surface of CHO cells. HHV-8 gB, gH, and gL could mediate the fusion of CHO cells with two different human cell types, embryonic kidney cells and B lymphocytes. Substituting gB(MUT) for gB significantly enhanced the fusion of CHO cells with human embryonic kidney cells but not B lymphocytes. Thus, two human cell types known to be susceptible to HHV-8 entry were also suitable targets for cell fusion induced by HHV-8 gB, gH, and gL. For human embryonic kidney cells and B cells at least, optimal fusion was noted with the expression of all three HHV-8 glycoproteins.


PROVIDER: S-EPMC155071 | BioStudies | 2002-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC3685210 | BioStudies
2016-01-01 | S-EPMC4970976 | BioStudies
2012-01-01 | S-EPMC3402990 | BioStudies
2015-01-01 | S-EPMC4473573 | BioStudies
2020-01-01 | S-EPMC7435202 | BioStudies
2018-01-01 | S-EPMC5809734 | BioStudies
2004-01-01 | S-EPMC536050 | BioStudies
2017-01-01 | S-EPMC5391448 | BioStudies
2008-01-01 | S-EPMC2447092 | BioStudies
2012-01-01 | S-EPMC3318624 | BioStudies