Dataset Information


Identification of iron-activated and -repressed Fur-dependent genes by transcriptome analysis of Neisseria meningitidis group B.

ABSTRACT: Iron is limiting in the human host, and bacterial pathogens respond to this environment by activating genes required for bacterial virulence. Transcriptional regulation in response to iron in Gram-negative bacteria is largely mediated by the ferric uptake regulator protein Fur, which in the presence of iron binds to a specific sequence in the promoter regions of genes under its control and acts as a repressor. Here we describe DNA microarray, computational and in vitro studies to define the Fur regulon in the human pathogen Neisseria meningitidis group B (strain MC58). After iron addition to an iron-depleted bacterial culture, 153 genes were up-regulated and 80 were down-regulated. Only 50% of the iron-regulated genes were found to contain Fur-binding consensus sequences in their promoter regions. Forty-two promoter regions were amplified and 32 of these were shown to bind Fur by gel-shift analysis. Among these genes, many of which had never been described before to be Fur-regulated, 10 were up-regulated on iron addition, demonstrating that Fur can also act as a transcriptional activator. Sequence alignment of the Fur-binding regions revealed that the N. meningitidis Fur-box encompasses the highly conserved (NATWAT)3 motif. Cluster analysis was effective in predicting Fur-regulated genes even if computer prediction failed to identify Fur-box-like sequences in their promoter regions. Microarray-generated gene expression profiling appears to be a very effective approach to define new regulons and regulatory pathways in pathogenic bacteria.

SUBMITTER: Grifantini R 

PROVIDER: S-EPMC170954 | BioStudies | 2003-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC1913314 | BioStudies
2010-02-11 | GSE20294 | GEO
1000-01-01 | S-EPMC2293247 | BioStudies
2008-01-01 | S-EPMC2346684 | BioStudies
2010-05-14 | E-GEOD-20294 | ArrayExpress
2010-01-01 | S-EPMC3068672 | BioStudies
1000-01-01 | S-EPMC177938 | BioStudies
1000-01-01 | S-EPMC3889615 | BioStudies
2017-01-01 | S-EPMC5400846 | BioStudies
2012-01-01 | S-EPMC3359417 | BioStudies