Dataset Information


Phosphate concentration regulates transcription of the Acinetobacter polyhydroxyalkanoic acid biosynthetic genes.

ABSTRACT: The polyhydroxyalkanoic acid (PHA) biosynthetic gene locus was cloned and characterized from an Acinetobacter sp. isolated from activated sludge. Nucleotide sequence analysis identified three clustered genes, phaAAc (encoding a beta-ketothiolase), phaBAc (encoding an acetoacetyl coenzyme A reductase), and phaCAc (encoding a PHA synthase). In addition, an open reading frame (ORF1) with potential to encode a 13-kDa protein was identified within this locus. The sequence of the putative translational product of ORF1 does not show significant similarity to any sequences in the database. A plasmid containing the Acinetobacter pha locus conferred the ability to accumulate poly-beta-hydroxybutyrate on its Escherichia coli host. These genes appear to lie in an operon transcribed by two promoters upstream of phaBAc, an apparent constitutive promoter, and a second promoter induced by phosphate starvation and under pho regulon control. These as well as a number of additional potential transcription start points were identified by a combination of primer extension and promoter-chloramphenicol acetyltransferase gene fusion studies carried out in Acinetobacter or E. coli transformants.


PROVIDER: S-EPMC177202 | BioStudies | 1995-01-01T00:00:00Z


REPOSITORIES: biostudies

Similar Datasets

1999-01-01 | S-EPMC91534 | BioStudies
1998-01-01 | S-EPMC107745 | BioStudies
2000-01-01 | S-EPMC92322 | BioStudies
2001-01-01 | S-EPMC95313 | BioStudies
1999-01-01 | S-EPMC93737 | BioStudies
1000-01-01 | S-EPMC176901 | BioStudies
2012-01-01 | S-EPMC3434029 | BioStudies
2001-01-01 | S-EPMC93259 | BioStudies
1998-01-01 | S-EPMC106744 | BioStudies
1973-01-01 | S-EPMC1177803 | BioStudies