Dataset Information


A crtB homolog essential for photochromogenicity in Mycobacterium marinum: isolation, characterization, and gene disruption via homologous recombination.

ABSTRACT: A gene essential for light-induced pigment production was isolated from the photochromogen Mycobacterium marinum by heterologous complementation of an M. marinum cosmid library in the nonchromogen Mycobacterium smegmatis. This gene is part of an operon and homologous to the Streptomyces griseus and Myxococcus xanthus crtB genes encoding phytoene synthase. Gene replacement at this locus was achieved via homologous recombination, demonstrating that its expression is essential for photochromogenicity. The ease of targeted gene disruption in this pathogenic Mycobacterium allows for the dissection of the molecular basis of mycobacterial pathogenesis.

SUBMITTER: Ramakrishnan L 

PROVIDER: S-EPMC179478 | BioStudies | 1997-01-01T00:00:00Z


REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC413336 | BioStudies
2017-01-01 | S-EPMC5989629 | BioStudies
2008-01-01 | S-EPMC2336800 | BioStudies
1000-01-01 | S-EPMC55297 | BioStudies
2018-01-01 | S-EPMC6089878 | BioStudies
2012-01-01 | S-EPMC3298165 | BioStudies
1998-01-01 | S-EPMC107510 | BioStudies
2019-01-01 | S-EPMC7025489 | BioStudies
1000-01-01 | S-EPMC3598387 | BioStudies
2004-01-01 | S-EPMC529147 | BioStudies