Unknown

Dataset Information

0

Inhibition of HPV-16 E6/E7 immortalization of normal keratinocytes by hairpin ribozymes.


ABSTRACT: HPV-16 E6 and E7 genes are required to efficiently immortalize a broad spectrum of cell types including cervical keratinocytes. Therefore, the E6/E7 genes can be considered relevant targets for anti-cancer therapy. We produced several engineered hairpin (HP) ribozymes to specifically disrupt HPV-16 E6/E7 mRNA. After extensive biochemical characterization, one anti-E6 HP ribozyme (R434) was selected for in vivo testing because of its superior catalytic capabilities. When expressed in cis, R434 efficiently inhibited E6 in vitro translation. Cis-expression of the HP ribozyme with HPV-16 E6/E7 genes in normal human keratinocytes reduced the growth rate and prevented immortalization. RNA analysis by reverse transcription-PCR showed that E6/E7 transcripts were cleaved in post-transfected cells and virtually were eliminated after long term expression. Of interest, an inactive version of the HP also was able to significantly affect the immortalizing ability of E6/E7, probably through passive hybridization. The combination of passive and cleaving antisense RNA therefore is established as an effective inhibitor of HPV-16 E6/E7 immortalization.

SUBMITTER: Alvarez-Salas LM 

PROVIDER: S-EPMC18715 | BioStudies | 1998-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC2773972 | BioStudies
2014-01-01 | S-EPMC4077794 | BioStudies
2019-01-01 | S-EPMC6452706 | BioStudies
2013-01-01 | S-EPMC3617164 | BioStudies
1000-01-01 | S-EPMC2744660 | BioStudies
2009-01-01 | S-EPMC2758704 | BioStudies
2014-06-26 | E-GEOD-58841 | ArrayExpress
1000-01-01 | S-EPMC5923196 | BioStudies
2018-01-01 | S-EPMC5833277 | BioStudies
1000-01-01 | S-EPMC506920 | BioStudies