Unknown

Dataset Information

0

Erwinia chrysanthemi tolC is involved in resistance to antimicrobial plant chemicals and is essential for phytopathogenesis.


ABSTRACT: TolC is the outer-membrane component of several multidrug resistance (MDR) efflux pumps and plays an important role in the survival and virulence of many gram-negative bacterial animal pathogens. We have identified and characterized the outer-membrane protein-encoding gene tolC in the bacterial plant pathogen Erwinia chrysanthemi EC16. The gene was found to encode a 51-kDa protein with 70% identity to its Escherichia coli homologue. The E. chrysanthemi gene was able to functionally complement the E. coli tolC gene with respect to its role in MDR efflux pumps. A tolC mutant of E. chrysanthemi was found to be extremely sensitive to antimicrobial agents, including several plant-derived chemicals. This mutant was unable to grow in planta and its ability to cause plant tissue maceration was severely compromised. The tolC mutant was shown to be defective in the efflux of berberine, a model antimicrobial plant chemical. These results suggest that by conferring resistance to the antimicrobial compounds produced by plants, the E. chrysanthemi tolC plays an important role in the survival and colonization of the pathogen in plant tissue.

SUBMITTER: Barabote RD 

PROVIDER: S-EPMC193971 | BioStudies | 2003-01-01

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6443642 | BioStudies
2015-01-01 | S-EPMC4402952 | BioStudies
2013-01-01 | S-EPMC3571319 | BioStudies
2018-01-01 | S-EPMC5840245 | BioStudies
2019-01-01 | S-EPMC6592562 | BioStudies
2019-01-01 | S-EPMC6425002 | BioStudies
2018-01-01 | S-EPMC6007937 | BioStudies
2008-01-01 | S-EPMC2292822 | BioStudies
2019-01-01 | S-EPMC6908949 | BioStudies
2011-01-01 | S-EPMC3126860 | BioStudies