Unknown

Dataset Information

0

Light chain editing generates polyreactive antibodies in chronic graft-versus-host reaction.


ABSTRACT: The chronic graft-versus-host (cGvH) reaction is a model of induced lupus caused by alloreactive CD4(+) T cells from a Bm-12 mouse in a C57BL/6 recipient. We used this cGvH reaction in C57BL/6 anti-DNA H chain transgenic mice, 56R/B6, to understand the structure, specificity, and origin of the induced autoantibodies (auto-Abs). We found anti-DNA Abs that reacted to several different antigens, such as phosphatidylserine, myelin basic protein, thyroglobulin, histone, insulin, cytochrome C, and beta-galactosidase. This polyreactivity was found for Abs from B cells that expressed the 56R H chain transgene with "editor" L chains that did not completely veto autoreactivity. We suggest that such incomplete editing results in polyreactivity and that incompletely edited polyreactive B cells influence the subsequent expression of pathogenic auto-Abs in disease. We also found B cells that coexpress kappa and lambda L chain. These B cells contributed to the autoimmune response and are possibly in the marginal zone of the spleen.

SUBMITTER: Witsch EJ 

PROVIDER: S-EPMC2118344 | BioStudies | 2006-01-01

REPOSITORIES: biostudies

Similar Datasets

2020-01-01 | S-EPMC7772752 | BioStudies
2015-01-01 | S-EPMC4595932 | BioStudies
2013-01-01 | S-EPMC3864117 | BioStudies
1000-01-01 | S-EPMC287323 | BioStudies
1993-01-01 | S-EPMC4627373 | BioStudies
2017-01-01 | S-EPMC5723652 | BioStudies
2012-01-01 | S-EPMC3402627 | BioStudies
2019-01-01 | S-EPMC6458971 | BioStudies
1000-01-01 | S-EPMC1297234 | BioStudies
2018-01-01 | S-EPMC5990490 | BioStudies