Dataset Information


A conserved function for a Caenorhabditis elegans Com1/Sae2/CtIP protein homolog in meiotic recombination.

ABSTRACT: Genome stability relies on faithful DNA repair both in mitosis and in meiosis. Here, we report on a Caenorhabditis elegans protein that we found to be homologous to the mammalian repair-related protein CtIP and to the budding yeast Com1/Sae2 recombination protein. A com-1 mutant displays normal meiotic chromosome pairing but forms irregular chromatin aggregates instead of diakinesis bivalents. While meiotic DNA double-strand breaks (DSBs) are formed, they appear to persist or undergo improper repair. Despite the presence of DSBs, the recombination protein RAD-51, which is known to associate with single-stranded DNA (ssDNA) flanking DSBs, does not localize to meiotic chromosomes in the com-1 mutant. Exposure of the mutant to gamma-radiation, however, induces RAD-51 foci, which suggests that the failure of RAD-51 to load is specific to meiotic (SPO-11-generated) DSBs. These results suggest that C. elegans COM-1 plays a role in the generation of ssDNA tails that can load RAD-51, invade homologous DNA tracts and thereby initiate recombination. Extrapolating from the worm homolog, we expect similar phenotypes for mutations in the mammalian tumor suppressor CtIP.


PROVIDER: S-EPMC2140103 | BioStudies | 2007-01-01

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3567172 | BioStudies
2018-01-01 | S-EPMC5948970 | BioStudies
1000-01-01 | S-EPMC5778493 | BioStudies
1000-01-01 | S-EPMC1069622 | BioStudies
2007-01-01 | S-EPMC2048528 | BioStudies
1000-01-01 | S-EPMC2807415 | BioStudies
2013-01-01 | S-EPMC3738457 | BioStudies
2007-01-01 | S-EPMC1933397 | BioStudies
1000-01-01 | S-EPMC4830875 | BioStudies
2011-01-01 | S-EPMC3169521 | BioStudies