Dataset Information


Shifts in targeting of class switch recombination sites in mice that lack mu switch region tandem repeats or Msh2.

ABSTRACT: The mechanisms that target class switch recombination (CSR) to antibody gene switch (S) regions are unknown. Analyses of switch site locations in wild-type mice and in mice that lack the Smu tandem repeats show shifts indicating that a 4-5-kb DNA domain (bounded upstream by the Imu promoter) is accessible for switching independent of Smu sequences. This CSR-accessible domain is reminiscent of the promoter-defined domains that target somatic hypermutation. Within the 4-5-kb CSR domain, the targeting of S site locations also depends on the Msh2 mismatch repair protein because Msh2-deficient mice show an increased focus of sites to the Smu tandem repeat region. We propose that Msh2 affects S site location because sequences with few activation-induced cytidine deaminase targets generate mostly switch DNA cleavages that require Msh2-directed processing to allow CSR joining.

PROVIDER: S-EPMC2212040 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC3677205 | BioStudies
| S-EPMC3645988 | BioStudies
| S-EPMC2442634 | BioStudies
| S-EPMC2137913 | BioStudies
| S-EPMC1952116 | BioStudies
| S-EPMC2194233 | BioStudies
2017-01-01 | S-EPMC5548798 | BioStudies
| S-EPMC2605234 | BioStudies
| S-EPMC3812156 | BioStudies
| S-EPMC4615597 | BioStudies