Unknown

Dataset Information

0

Identification and characterization of a human herpesvirus 6 gene segment capable of transactivating the human immunodeficiency virus type 1 long terminal repeat in an Sp1 binding site-dependent manner.


ABSTRACT: The human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) is transactivated by various extracellular signals and viral cofactors that include human herpesviruses. These transactivators are capable of transactivating the HIV-1 LTR through the transactivation response element, NF-kappa B, or other regulatory binding elements. Human herpesvirus 6 (HHV-6) is a potential cofactor of HIV-1. Here, we report that an HHV-6 gene segment, ZVH14, which can neoplastically transform NIH 3T3 and human keratinocytes, is capable of transactivating HIV-1 LTR chloramphenicol acetyltransferase constructs in an Sp1 binding site-dependent manner. Transactivation increased synergistically in the presence of multiple Sp1 sites and was dramatically reduced by cotransfection with oligomers designed to form triplex structures with HIV-1 LTR Sp1 binding sites. HIV-1 LTR NF-kappa B sites were not essential for ZVH14-mediated transactivation. A putative open reading frame in ZVH14, B115, which may encode a highly basic peptide consisting of 115 amino acid residues, showed transactivation capacity similar to that of ZVH14. This open reading frame also transactivated the HIV-1 LTR in an Sp1 site-dependent fashion in African green monkey kidney cells and human T cells. These data suggest that HHV-6 may stimulate HIV-1 replication via transactivation of Sp1 binding sites present in the HIV-1 promoter.

PROVIDER: S-EPMC236630 | BioStudies | 1994-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC249019 | BioStudies
2013-01-01 | S-EPMC3708878 | BioStudies
2000-01-01 | S-EPMC113184 | BioStudies
2009-01-01 | S-EPMC2607019 | BioStudies
2000-01-01 | S-EPMC111760 | BioStudies
1999-01-01 | S-EPMC103872 | BioStudies
1997-01-01 | S-EPMC1218751 | BioStudies
2009-01-01 | S-EPMC2663287 | BioStudies
1000-01-01 | S-EPMC236807 | BioStudies
2020-01-01 | S-EPMC7081896 | BioStudies