Unknown

Dataset Information

0

Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.


ABSTRACT: Virally transduced genes are often silenced after integration into the host genome. Chromatin immunoprecipitation and nuclease sensitivity experiments now demonstrate that silencing of the transgene is characterized by deacetylation of histone H4 lysines and chromatin condensation. Trichostatin A treatment results in dramatic reactivation of gene expression that is preceded by histone acetylation and chromatin decondensation. Analysis of individual histone H4 lysines demonstrate that chromatin domain opening is coincident with rapid acetylation of histone H4 K5, K12, and K16 and that maintenance of the open domain is correlated with acetylation of histone H4 K8. Removal of trichostatin A results in rapid deacetylation of histone H4 K8, chromatin condensation, and transcription silencing. The results suggest that deacetylation of histone H4 lysines and coincident chromatin condensation are critically involved in the silencing of virally transduced genes.

SUBMITTER: Chen WY 

PROVIDER: S-EPMC26671 | BioStudies | 2000-01-01

REPOSITORIES: biostudies

Similar Datasets

2009-01-01 | S-EPMC2667965 | BioStudies
1000-01-01 | S-EPMC3095308 | BioStudies
1000-01-01 | S-EPMC2172711 | BioStudies
2012-01-01 | S-EPMC3445983 | BioStudies
1000-01-01 | S-EPMC2585588 | BioStudies
2011-01-01 | S-EPMC3061077 | BioStudies
2003-01-01 | S-EPMC164624 | BioStudies
1998-01-01 | S-EPMC109097 | BioStudies
2014-01-01 | S-EPMC3986647 | BioStudies
2005-01-01 | S-EPMC2749475 | BioStudies