Unknown

Dataset Information

0

Mechanisms that specify promoter nucleosome location and identity.


ABSTRACT: The chromatin architecture of eukaryotic gene promoters is generally characterized by a nucleosome-free region (NFR) flanked by at least one H2A.Z variant nucleosome. Computational predictions of nucleosome positions based on thermodynamic properties of DNA-histone interactions have met with limited success. Here we show that the action of the essential RSC remodeling complex in S. cerevisiae helps explain the discrepancy between theory and experiment. In RSC-depleted cells, NFRs shrink such that the average positions of flanking nucleosomes move toward predicted sites. Nucleosome positioning at distinct subsets of promoters additionally requires the essential Myb family proteins Abf1 and Reb1, whose binding sites are enriched in NFRs. In contrast, H2A.Z deposition is dispensable for nucleosome positioning. By regulating H2A.Z deposition using a steroid-inducible protein splicing strategy, we show that NFR establishment is necessary for H2A.Z deposition. These studies suggest an ordered pathway for the assembly of promoter chromatin architecture.

SUBMITTER: Hartley PD 

PROVIDER: S-EPMC2677553 | BioStudies | 2009-01-01

REPOSITORIES: biostudies

Similar Datasets

2009-05-01 | GSE13446 | GEO
2009-04-30 | E-GEOD-13446 | ArrayExpress
2016-01-01 | S-EPMC5240917 | BioStudies
2020-01-01 | S-EPMC7041490 | BioStudies
2005-01-01 | S-EPMC2039754 | BioStudies
1000-01-01 | S-EPMC4233242 | BioStudies
2010-01-01 | S-EPMC2922504 | BioStudies
2009-01-01 | S-EPMC2648986 | BioStudies
2016-08-14 | E-GEOD-72106 | ArrayExpress
1000-01-01 | S-EPMC3698015 | BioStudies