Unknown

Dataset Information

0

Antibodies in a heavy chain knock-in mouse exhibit characteristics of early heavy chain rearrangement.


ABSTRACT: Studies in autoantibody transgenic mice have demonstrated receptor editing rearrangements at Ab H and L chain loci. However, the physiologic role of H chain editing (V(H) replacement and rearrangement on the second allele) has been called into question. It is unclear if additional rounds of H chain rearrangement are driven by BCR specificity. In this study, we analyze the manner in which B cells undergo additional H chain rearrangements in an anti-DNA H chain knock-in mouse, B6.56R. We find that rearrangements in 56R(+) B cells tend to involve the D gene locus on both alleles and the most J(H)-proximal V(H) gene segments on the endogenous allele. As a result, some B cells exhibit V(D)J rearrangements on both H chain alleles, yet allelic exclusion is tightly maintained in mature 56R B cells. As B cells mature, a higher proportion expresses the nontransgenic H chain allele. Rearrangements on both H chain alleles exhibit junctional diversity consistent with TdT-mediated N-addition, and TdT RNA is expressed exclusively at the pro-B cell stage in B6.56R. Collectively, these findings favor a single, early window of H chain rearrangement in B6.56R that precedes the expression of a functional BCR. B cells that happen to successfully rearrange another H chain may be favored in the periphery.

SUBMITTER: Yunk L 

PROVIDER: S-EPMC2700953 | BioStudies | 2009-01-01

REPOSITORIES: biostudies

Similar Datasets

2006-01-01 | S-EPMC1544076 | BioStudies
2014-01-01 | S-EPMC4053206 | BioStudies
1000-01-01 | S-EPMC4321307 | BioStudies
1000-01-01 | S-EPMC3127927 | BioStudies
1000-01-01 | S-EPMC4351640 | BioStudies
2003-01-01 | S-EPMC196178 | BioStudies
2013-01-01 | S-EPMC3737562 | BioStudies
2017-01-01 | S-EPMC5728594 | BioStudies
1000-01-01 | S-EPMC2230400 | BioStudies
| GSE47128 | GEO