Dataset Information


Spin trapping and cytoprotective properties of fluorinated amphiphilic carrier conjugates of cyclic versus linear nitrones.

ABSTRACT: Nitrones have been employed as spin trapping reagent as well as pharmacological agent against neurodegenerative diseases and ischemia-reperfusion induced injury. The structure-activity relationship was explored for the two types of nitrones, i.e., cyclic (DMPO) and linear (PBN), which are conjugated to a fluorinated amphiphilic carrier (FAC) for their cytoprotective properties against hydrogen peroxide (H(2)O(2)), 3-morpholinosynonimine hydrochloride (SIN-1), and 4-hydroxynonenal (HNE) induced cell death on bovine aortic endothelial cells. The compound FAMPO was synthesized and characterized, and its physical-chemical and spin trapping properties were explored. Cytotoxicity and cytoprotective properties of various nitrones either conjugated and nonconjugated to FAC (i.e., AMPO, FAMPO, PBN, and FAPBN) were assessed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) reduction assay. Results show that of all the nitrones tested, FAPBN is the most protective against H(2)O(2), but FAMPO and to a lesser extent its unconjugated form, AMPO, are more protective against SIN-1 induced cytotoxicity. However, none of the nitrones used protect the cells from HNE-induced cell death. The difference in the cytoprotective properties observed between the cyclic and linear nitrones may arise from the differences in their intrinsic antioxidant properties and localization in the cell.


PROVIDER: S-EPMC2748169 | BioStudies | 2009-01-01

REPOSITORIES: biostudies

Similar Datasets

2012-01-01 | S-EPMC3428044 | BioStudies
2019-01-01 | S-EPMC6545623 | BioStudies
2010-01-01 | S-EPMC2818661 | BioStudies
1982-01-01 | S-EPMC1158389 | BioStudies
2016-01-01 | S-EPMC5248535 | BioStudies
2007-01-01 | S-EPMC2544612 | BioStudies
2010-01-01 | S-EPMC2916040 | BioStudies
2020-01-01 | S-EPMC7447640 | BioStudies
2020-01-01 | S-EPMC7663103 | BioStudies
1000-01-01 | S-EPMC3173208 | BioStudies