Unknown

Dataset Information

5

RhBMP-2 modulation of gene expression in infected segmental bone defects.


ABSTRACT: The osteoinductive capability of BMPs appears diminished in the setting of acute infection. We applied rhBMP-2 to a segmental defect in a rat femur and measured the expression of key bone formation genes in the presence of acute infection. Types I and II collagen, osteocalcin, and BMP Type II receptor mRNA expression were characterized in 72 Sprague-Dawley rats, which received either bovine collagen carrier with 200 mug rhBMP-2 plus Staphylococcus aureus, carrier with bacteria only, carrier with rhBMP-2 only, or carrier alone. Six animals from each group were euthanized at 1, 2, and 4 weeks. Total RNA was isolated and extracted, and mRNA was determined by real-time comparative quantitative PCR. Infected defects had little expression of collagen I and II and osteocalcin mRNAs, while BMP receptor II expression with infection was greater than carrier-only controls at weeks 2 and 4. Notably, all four genes were upregulated in infected defects in the presence of rhBMP-2. Thus, in a clinical setting with a high risk of infection and nonunion, such as a compound fracture with bone loss, rhBMP-2 may increase the rate and extent of bone formation. Even if infection does occur, rhBMP-2 may allow a quicker overall recovery time.

SUBMITTER: Brick KE 

PROVIDER: S-EPMC2772904 | BioStudies | 2009-01-01

SECONDARY ACCESSION(S): 10.1007/s11999-008-0599-3

REPOSITORIES: biostudies

altmetric image

Publications

rhBMP-2 modulation of gene expression in infected segmental bone defects.

Brick Katherine E KE   Chen Xinqian X   Lohr Jamie J   Schmidt Andrew H AH   Kidder Louis S LS   Lew William D WD  

Clinical orthopaedics and related research 20081119 12


The osteoinductive capability of BMPs appears diminished in the setting of acute infection. We applied rhBMP-2 to a segmental defect in a rat femur and measured the expression of key bone formation genes in the presence of acute infection. Types I and II collagen, osteocalcin, and BMP Type II receptor mRNA expression were characterized in 72 Sprague-Dawley rats, which received either bovine collagen carrier with 200 mug rhBMP-2 plus Staphylococcus aureus, carrier with bacteria only, carrier with  ...[more]

Similar Datasets

2019-01-01 | S-EPMC6804013 | BioStudies
1000-01-01 | S-EPMC4086523 | BioStudies
2016-01-01 | S-EPMC5457269 | BioStudies
2016-01-01 | S-EPMC4903101 | BioStudies
2014-01-01 | S-EPMC4894319 | BioStudies
2012-01-01 | S-EPMC4111513 | BioStudies
2016-01-01 | S-EPMC5134101 | BioStudies
2014-01-01 | S-EPMC4397773 | BioStudies
2018-01-01 | S-EPMC5773187 | BioStudies
2014-01-01 | S-EPMC3913585 | BioStudies