Unknown

Dataset Information

0

CD38/cADPR/Ca2+ pathway promotes cell proliferation and delays nerve growth factor-induced differentiation in PC12 cells.


ABSTRACT: Intracellular Ca(2+) mobilization plays an important role in a wide variety of cellular processes, and multiple second messengers are responsible for mediating intracellular Ca(2+) changes. Here we explored the role of one endogenous Ca(2+)-mobilizing nucleotide, cyclic adenosine diphosphoribose (cADPR), in the proliferation and differentiation of neurosecretory PC12 cells. We found that cADPR induced Ca(2+) release in PC12 cells and that CD38 is the main ADP-ribosyl cyclase responsible for the acetylcholine (ACh)-induced cADPR production in PC12 cells. In addition, the CD38/cADPR signaling pathway is shown to be required for the ACh-induced Ca(2+) increase and cell proliferation. Inhibition of the pathway, on the other hand, accelerated nerve growth factor (NGF)-induced neuronal differentiation in PC12 cells. Conversely, overexpression of CD38 increased cell proliferation but delayed NGF-induced differentiation. Our data indicate that cADPR plays a dichotomic role in regulating proliferation and neuronal differentiation of PC12 cells.

PROVIDER: S-EPMC2785564 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC3471724 | BioStudies
| S-EPMC4784992 | BioStudies
| S-EPMC5555186 | BioStudies
| S-EPMC4454144 | BioStudies
| S-EPMC3247979 | BioStudies
| S-EPMC5821947 | BioStudies
| S-EPMC4734301 | BioStudies
2004-01-01 | S-EPMC533971 | BioStudies
| S-EPMC2898418 | BioStudies
| S-EPMC4370261 | BioStudies