Dataset Information


SaeR binds a consensus sequence within virulence gene promoters to advance USA300 pathogenesis.

ABSTRACT: This investigation examines the role of the SaeR/S 2-component system in USA300, a prominent circulating clone of community-associated methicillin-resistant Staphylococcus aureus. Using a saeR/S isogenic deletion mutant of USA300 (USA300DeltasaeR/S) in murine models of sepsis and soft-tissue infection revealed that this sensory system is critical to pathogenesis of USA300 during both superficial and invasive infection. Oligonucleotide microarray and real-time reverse-transcriptase polymerase chain reaction identified numerous extracellular virulence genes that are down-regulated in USA300DeltasaeR/S. Unexpectedly, an up-regulation of mecA and mecR1 corresponded to increased methicillin resistance in USA300DeltasaeR/S. 5'-RACE analysis defined transcript start sites for sbi, efb, mecA, lukS-PV, hlb, SAUSA300_1975, and hla, to underscore a conserved consensus sequence within promoter regions of genes under strong SaeR/S transcriptional regulation. Electrophoretic mobility shift assay experiments illustrated direct binding of SaeR(His) to promoter regions containing the conserved consensus sequence. Collectively, the findings of this investigation demonstrate that SaeR/S directly interacts with virulence gene promoters to significantly influence USA300 pathogenesis.


PROVIDER: S-EPMC2798008 | BioStudies | 2010-01-01

REPOSITORIES: biostudies

Similar Datasets

2018-01-01 | S-EPMC7191620 | BioStudies
2014-01-01 | S-EPMC4435966 | BioStudies
2004-01-01 | S-EPMC478485 | BioStudies
2018-01-01 | S-EPMC6302044 | BioStudies
2001-01-01 | S-EPMC90585 | BioStudies
2016-01-01 | S-EPMC4694005 | BioStudies
2019-01-01 | S-EPMC7187620 | BioStudies
2019-01-01 | S-EPMC6813604 | BioStudies
2012-01-01 | S-EPMC3406092 | BioStudies
1000-01-01 | S-EPMC5069094 | BioStudies