Unknown

Dataset Information

0

IL-17 produced by neutrophils regulates IFN-gamma-mediated neutrophil migration in mouse kidney ischemia-reperfusion injury.


ABSTRACT: The IL-23/IL-17 and IL-12/IFN-gamma cytokine pathways have a role in chronic autoimmunity, which is considered mainly a dysfunction of adaptive immunity. The extent to which they contribute to innate immunity is, however, unknown. We used a mouse model of acute kidney ischemia-reperfusion injury (IRI) to test the hypothesis that early production of IL-23 and IL-12 following IRI activates downstream IL-17 and IFN-gamma signaling pathways and promotes kidney inflammation. Deficiency in IL-23, IL-17A, or IL-17 receptor (IL-17R) and mAb neutralization of CXCR2, the p19 subunit of IL-23, or IL-17A attenuated neutrophil infiltration in acute kidney IRI in mice. We further demonstrate that IL-17A produced by GR-1+ neutrophils was critical for kidney IRI in mice. Activation of the IL-12/IFN-gamma pathway and NKT cells by administering alpha-galactosylceramide-primed bone marrow-derived DCs increased IFN-gamma production following moderate IRI in WT mice but did not exacerbate injury or enhance IFN-gamma production in either Il17a-/- or Il17r-/- mice, which suggested that IL-17 signaling was proximal to IFN-gamma signaling. This was confirmed by the finding that IFN-gamma administration reversed the protection seen in Il17a-/- mice subjected to IRI, whereas IL-17A failed to reverse protection in Ifng-/- mice. These results demonstrate that the innate immune component of kidney IRI requires dual activation of the IL-12/IFN-gamma and IL-23/IL-17 signaling pathways and that neutrophil production of IL-17A is upstream of IL-12/IFN-gamma. These mechanisms might contribute to reperfusion injury in other organs.

SUBMITTER: Li L 

PROVIDER: S-EPMC2798679 | BioStudies | 2010-01-01

REPOSITORIES: biostudies

Similar Datasets

2016-01-01 | S-EPMC4785101 | BioStudies
1000-01-01 | S-EPMC3003524 | BioStudies
2009-01-01 | S-EPMC2759196 | BioStudies
2020-01-01 | S-EPMC7567645 | BioStudies
2009-01-01 | S-EPMC3607320 | BioStudies
2018-01-01 | S-EPMC5875946 | BioStudies
2013-01-01 | S-EPMC3770803 | BioStudies
2009-01-01 | S-EPMC3594767 | BioStudies
2020-01-01 | S-EPMC7067553 | BioStudies
2009-01-01 | S-EPMC2767186 | BioStudies