Unknown

Dataset Information

0

Lipid-dependent cytotoxicity by the lipase PLRP2 and by PLRP2-positive cytotoxic T lymphocytes (CTLs).


ABSTRACT: IL-4 induces a lipase, pancreatic lipase related protein 2 (PLRP2), in cytotoxic T lymphocytes (CTLs). Because PLRP2 in semen can mediate lipid-dependent toxicity to sperm, we questioned whether CTL-derived PLRP2 could support similar cytotoxicity toward tumor cells. Recombinant PLRP2 was toxic to P815 tumor cells in 48 h when lipid and another protein, colipase, were present. However, PLRP2-positive CTLs (induced with many lots of IL-4) were unable to mediate lipid-dependent cytotoxicity. Notably, CTLs induced with only one lot of IL-4 had lipid-dependent cytotoxicity. The exceptional lot of IL-4 was effective in multiple experiments at inducing lipid-dependent cytotoxicity. The lipid-dependent cytotoxicity it induced was determined to be perforin-independent. CTLs induced with IL-4 that was unable to induce lipid-dependent cytotoxicity had mRNA for PLRP2 but not mRNA for colipase. Therefore, we added exogenous colipase to the CTL assays but still cytotoxicity was unchanged. We conclude (1) that lipid-dependent cytotoxicity, promoted by the lipase PLRP2 and colipase, will kill tumor cells and (2) that more than PLRP2 alone is required for lipid-dependent cytotoxicity mediated by CTLs.

SUBMITTER: Alves BN 

PROVIDER: S-EPMC2837949 | BioStudies | 2009-01-01

REPOSITORIES: biostudies

Similar Datasets

2009-01-01 | S-EPMC2735286 | BioStudies
2019-01-01 | S-EPMC6814837 | BioStudies
1000-01-01 | S-EPMC5645350 | BioStudies
2017-01-01 | S-EPMC6999238 | BioStudies
1000-01-01 | S-EPMC4156562 | BioStudies
1999-01-01 | S-EPMC1220197 | BioStudies
2020-01-01 | S-EPMC7094207 | BioStudies
2017-01-01 | S-EPMC5203973 | BioStudies
1000-01-01 | S-EPMC4348954 | BioStudies
2013-12-02 | E-GEOD-51393 | ArrayExpress