Dataset Information


Comparison of radioimmunoprecipitation with luciferase immunoprecipitation for autoantibodies to GAD65 and IA-2beta.

ABSTRACT: To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2beta.Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2beta.LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2beta by LIPS and RIP were not statistically different.The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.


PROVIDER: S-EPMC2845022 | BioStudies | 2010-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2008-01-01 | S-EPMC2215321 | BioStudies
1000-01-01 | S-EPMC1347964 | BioStudies
2009-01-01 | S-EPMC2735370 | BioStudies
2015-01-01 | S-EPMC4564997 | BioStudies
2009-01-01 | S-EPMC2672562 | BioStudies
2003-01-01 | S-EPMC1223505 | BioStudies
1000-01-01 | S-EPMC4468815 | BioStudies
2015-01-01 | S-EPMC4566937 | BioStudies
2010-01-01 | S-EPMC2877104 | BioStudies
2015-01-01 | S-EPMC5680538 | BioStudies