Dataset Information


Direct interactions of intraflagellar transport complex B proteins IFT88, IFT52, and IFT46.

ABSTRACT: Intraflagellar transport (IFT) particles of Chlamydomonas reinhardtii contain two distinct protein complexes, A and B, composed of at least 6 and 15 protein subunits, respectively. As isolated from C. reinhardtii flagella, IFT complex B can be further reduced to a approximately 500-kDa core that contains IFT88, 2x IFT81, 2x IFT74/72, IFT52, IFT46, IFT27, IFT25, and IFT22. In this study, yeast-based two-hybrid analysis was combined with bacterial coexpression to show that three of the core B subunits, IFT88, IFT52, and IFT46, interact directly with each other and, together, are capable of forming a ternary complex. Chemical cross-linking results support the IFT52-IFT88 interaction and provide additional evidence of an association between IFT27 and IFT81. With previous studies showing that IFT81 and IFT74/72 interact to form a (IFT81)(2)(IFT74/72)(2) heterotetramer and that IFT27 and IFT25 form a heterodimer, the architecture of complex B is revealing itself. Last, electroporation of recombinant IFT46 was used to rescue flagellar assembly of a newly identified ift46 mutant and to monitor in vivo localization and movement of the IFT particles.


PROVIDER: S-EPMC2898376 | BioStudies | 2010-01-01


REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5694392 | BioStudies
2009-01-01 | S-EPMC2671599 | BioStudies
2019-01-01 | S-EPMC6614581 | BioStudies
1000-01-01 | S-EPMC3143597 | BioStudies
2016-01-01 | S-EPMC5506485 | BioStudies
2016-01-01 | S-EPMC5291235 | BioStudies
2017-01-01 | S-EPMC6355109 | BioStudies
1000-01-01 | S-EPMC5703605 | BioStudies
2014-01-01 | S-EPMC4210449 | BioStudies
2013-01-01 | S-EPMC4359902 | BioStudies