Dataset Information


Structure of duplex DNA containing the cisplatin 1,2-{Pt(NH3)2}2+-d(GpG) cross-link at 1.77 A resolution.

ABSTRACT: We report the 1.77-A resolution X-ray crystal structure of a dodecamer DNA duplex with the sequence 5'-CCTCTGGTCTCC-3' that has been modified to contain a single engineered 1,2-cis-{Pt(NH(3))(2)}(2+)-d(GpG) cross-link, the major DNA adduct of cisplatin. These data represent a significant improvement in resolution over the previously published 2.6-A structure. The ammine ligands in this structure are clearly resolved, leading to improved visualization of the cross-link geometry with respect to both the platinum center and to the nucleobases, which adopt a higher energy conformation. Also better resolved are the deoxyribose sugar puckers, which allow us to re-examine the global structure of platinum-modified DNA. Another new feature of this model is the location of four octahedral [Mg(H(2)O)(6)](2+) ions associated with bases in the DNA major groove and the identification of 124 ordered water molecules that participate in hydrogen-bonding interactions with either the nucleic acid or the diammineplatinum(II) moiety.

PROVIDER: S-EPMC2917232 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC6657808 | BioStudies
| S-EPMC4689412 | BioStudies
| S-EPMC5137628 | BioStudies
| S-EPMC4150612 | BioStudies
| S-EPMC2912421 | BioStudies
| S-EPMC3625460 | BioStudies
| S-EPMC1431574 | BioStudies
2016-01-01 | S-EPMC4992897 | BioStudies
2013-01-01 | S-EPMC3610147 | BioStudies
| S-EPMC4645071 | BioStudies