Unknown

Dataset Information

0

Molecular mechanisms of simian immunodeficiency virus SIV(agm) RNA encapsidation.


ABSTRACT: Primate lentiviruses are composed of several distinct lineages, including human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus SIVagm. HIV-1 and HIV-2 have significant differences in the mechanisms of viral RNA encapsidation. Therefore, the RNA packaging mechanisms of SIVagm cannot be predicted from the studies of HIV-1 and HIV-2. We examined the roles of the nucleocapsid (NC) zinc finger motifs on RNA packaging by mutating the conserved zinc finger (CCHC) motifs, and whether SIVagm has a preference to package RNA in cis by comparing the RNA packaging efficiencies of gag mutants in the presence of a wild-type vector. Our results indicate that the SIVagm NC domain plays an important role in Gag-RNA recognition; furthermore SIVagm is distinct from the other currently known primate lentiviruses as destroying either zinc finger motif in the NC causes very drastic RNA packaging defects. Additionally, trans-packaging is a major mechanism for SIVagm RNA encapsidation.

SUBMITTER: Fu W 

PROVIDER: S-EPMC2920611 | BioStudies | 2007-01-01

REPOSITORIES: biostudies

Similar Datasets

2005-01-01 | S-EPMC1143677 | BioStudies
2008-01-01 | S-EPMC2224418 | BioStudies
1000-01-01 | S-EPMC189545 | BioStudies
2014-01-01 | S-EPMC3911680 | BioStudies
2015-01-01 | S-EPMC4295846 | BioStudies
2010-01-01 | S-EPMC2903278 | BioStudies
1000-01-01 | S-EPMC3014162 | BioStudies
1000-01-01 | S-EPMC3131246 | BioStudies
2013-01-01 | S-EPMC3587049 | BioStudies
2003-01-01 | S-EPMC187409 | BioStudies