Dataset Information


MicroRNA expression in Sezary syndrome: identification, function, and diagnostic potential.

ABSTRACT: MicroRNAs are commonly aberrantly expressed in many cancers. Very little is known of their role in T-cell lymphoma, however. We therefore elucidated the complete miRNome of purified T cells from 21 patients diagnosed with Sézary Syndrome (SzS), a rare aggressive primary cutaneous T-cell (CD4(+)) lymphoma. Unsupervised cluster analysis of microarray data revealed that the microRNA expression profile was distinct from CD4(+) T-cell controls and B-cell lymphomas. The majority (104 of 114) of SzS-associated microRNAs (P < .05) were down-regulated and their expression pattern was largely consistent with previously reported genomic copy number abnormalities and were found to be highly enriched (P < .001) for aberrantly expressed target genes. Levels of miR-223 distinguished SzS samples (n = 32) from healthy controls (n = 19) and patients with mycosis fungoides (n = 11) in more than 90% of samples. Furthermore, we demonstrate that the down-regulation of intronically encoded miR-342 plays a role in the pathogenesis of SzS by inhibiting apoptosis, and describe a novel mechanism of regulation for this microRNA via binding of miR-199a* to its host gene. We also provide the first in vivo evidence for down-regulation of the miR-17-92 cluster in malignancy and demonstrate that ectopic miR-17-5p expression increases apoptosis and decreases cell proliferation in SzS cells.

PROVIDER: S-EPMC2938132 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| E-GEOD-21697 | BioStudies
2010-05-12 | GSE21697 | GEO
2010-05-12 | E-GEOD-21697 | ArrayExpress
| S-EPMC7295742 | BioStudies
| S-EPMC3012540 | BioStudies
2017-01-01 | S-EPMC5581081 | BioStudies
| E-GEOD-24371 | BioStudies
| S-EPMC3790944 | BioStudies
| S-EPMC3347661 | BioStudies
2011-01-01 | S-EPMC3120802 | BioStudies