Dataset Information


A three-dimensional gel bioreactor for assessment of cardiomyocyte induction in skeletal muscle-derived stem cells.

ABSTRACT: Skeletal muscle-derived stem cells (MDSCs) are able to differentiate into cardiomyocytes (CMs). However, it remains to be investigated whether differentiated CMs contract similar to native CMs. Here, we developed a three-dimensional collagen gel bioreactor (3DGB) that induces a working CM phenotype from MDSCs, and the contractile properties are directly measured as an engineered cardiac tissue. Neonate rat MDSCs were isolated from hind-leg muscles via the preplate technique. Isolated MDSCs were approximately 60% positive to Sca-1 and negative to CD34, CD45, or c-kit antigens. We sorted Sca-1(-) MDSCs and constructed MDSC-3DGBs by mixing MDSCs with acid soluble rat tail collagen type-I and matrix factors. MDSC-3DGB exhibited spontaneous cyclic contraction by culture day 7. MDSC-3DGB expressed cardiac-specific genes and proteins. Histological assessment revealed that cardiac-specific troponin-T and -I expressed in a typical striation pattern and connexin-43 was expressed similar to the native fetal ventricular papillary muscle. beta-Adrenergic stimulation increased MDSC-3DGB spontaneous beat frequency. MDSC-3DGB generated contractile force and intracellular calcium ion transients similar to engineered cardiac tissue from native cardiac cells. Results suggest that MDSC-3DGB induces a working CM phenotype in MDSCs and is a useful 3D culture system to directly assess the contractile properties of differentiated CMs in vitro.


PROVIDER: S-EPMC2945363 | BioStudies | 2010-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-01-01 | S-EPMC4196107 | BioStudies
1000-01-01 | S-EPMC4840925 | BioStudies
1000-01-01 | S-EPMC3267970 | BioStudies
2018-01-01 | S-EPMC5934578 | BioStudies
2013-01-01 | S-EPMC4886501 | BioStudies
1000-01-01 | S-EPMC4046249 | BioStudies
2016-01-01 | S-EPMC6581838 | BioStudies
2017-01-01 | S-EPMC5558787 | BioStudies
2014-01-01 | S-EPMC4101664 | BioStudies
2015-01-01 | S-EPMC4670592 | BioStudies