Unknown

Dataset Information

5

Phenotypic characterization of Corynebacterium glutamicum using elementary modes towards synthesis of amino acids.


ABSTRACT: UNLABELLED: Elementary flux mode (EFM) analysis is a powerful tool to represent the metabolic network structure and can be further utilized for flux analysis. The method enables characterization and quantification of feasible phenotypes in microbes. EFM analysis was employed to characterize the phenotype of Corynebacterium glutamicum to yield various amino acids. The metabolic network of C. glutamicum yielded 62 elementary modes by incorporating the accumulation of amino acids namely, lysine, alanine, valine, glutamine and glutamate. The analysis also allowed us to compute the maximum theoretical yield for the synthesis of various amino acids. These 62 elementary modes were further used to obtain optimal phenotypic space towards accumulation of biomass and lysine. The study indicated that the optimal solution space from 62 elementary modes forms a super space which incorporates various mutants including lysine producing strain of C. glutamicum. The analysis was also extended to obtain sensitivity of the network to variation in the stoichiometry of NADP in the definition of biomass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11693-011-9073-8) contains supplementary material, which is available to authorized users.

SUBMITTER: Radhakrishnan D 

PROVIDER: S-EPMC3065593 | BioStudies | 2010-01-01T00:00:00Z

REPOSITORIES: biostudies

altmetric image

Publications

Phenotypic characterization of Corynebacterium glutamicum using elementary modes towards synthesis of amino acids.

Radhakrishnan Devesh D   Rajvanshi Meghna M   Venkatesh K V KV  

Systems and synthetic biology 20101201 4


<h4>Unlabelled</h4>Elementary flux mode (EFM) analysis is a powerful tool to represent the metabolic network structure and can be further utilized for flux analysis. The method enables characterization and quantification of feasible phenotypes in microbes. EFM analysis was employed to characterize the phenotype of Corynebacterium glutamicum to yield various amino acids. The metabolic network of C. glutamicum yielded 62 elementary modes by incorporating the accumulation of amino acids namely, lys  ...[more]

Similar Datasets

1000-01-01 | S-EPMC1617123 | BioStudies
1000-01-01 | S-EPMC3148577 | BioStudies
1000-01-01 | S-EPMC3750108 | BioStudies
2013-01-01 | S-EPMC3812217 | BioStudies
2020-01-01 | S-EPMC7074156 | BioStudies
1000-01-01 | S-EPMC4029027 | BioStudies
1000-01-01 | S-EPMC6330010 | BioStudies
2011-01-01 | S-EPMC3067661 | BioStudies
2015-01-01 | S-EPMC4475075 | BioStudies
2015-01-01 | S-EPMC4388735 | BioStudies