BackgroundFor the detection of allergen-specific IgE in sera, solid-phase IgE-binding assays like the CAP test are commonly used. Although such immunochemical methods are very sensitive, they frequently produce false positives. Degranulation of the human IgE receptor (Fc?RI)-transfected rat mast cell (RBL) lines seems to be a possible indicator for human IgE, but spontaneous mediator release from these cells in the presence of human sera is not negligible.
MethodsThe nuclear factor of activated T-cells (NFAT)-responsive luciferase reporter gene was stably transfected into human Fc?RI-expressing RBL-SX38 cells. One established clone (RS-ATL8) was sensitized with 1?:?100 dilution of sera from patients with egg white allergy and then stimulated with purified or a crude extract of egg white allergen.
ResultsSensitization with 15?pg/ml IgE was sufficient to detect IgE crosslinking-induced luciferase expression (EXiLE) by anti-IgE stimulation. Allergen-specific EXiLE was elicited by as little as 1?fg/ml of egg white protein without cytotoxicity. There was a good correlation between results with EXiLE and oral food challenge tests on patients with egg allergy (P?=?0.001687, Fisher's exact test). The measured values of EXiLE and the CAP test also correlated well (R?=?0.9127, Spearman's test).
ConclusionThe EXiLE test using RS-ATL8 cells is a promising in vitro IgE test to evaluate the biological activity of the binding between IgE and allergens.
SUBMITTER: Nakamura R