Dataset Information


Single-molecule paleoenzymology probes the chemistry of resurrected enzymes.

ABSTRACT: It is possible to travel back in time at the molecular level by reconstructing proteins from extinct organisms. Here we report the reconstruction, based on sequence predicted by phylogenetic analysis, of seven Precambrian thioredoxin enzymes (Trx) dating back between ~1.4 and ~4 billion years (Gyr). The reconstructed enzymes are up to 32 °C more stable than modern enzymes, and the oldest show markedly higher activity than extant ones at pH 5. We probed the mechanisms of reduction of these enzymes using single-molecule force spectroscopy. From the force dependency of the rate of reduction of an engineered substrate, we conclude that ancient Trxs use chemical mechanisms of reduction similar to those of modern enzymes. Although Trx enzymes have maintained their reductase chemistry unchanged, they have adapted over 4 Gyr to the changes in temperature and ocean acidity that characterize the evolution of the global environment from ancient to modern Earth.

SUBMITTER: Perez-Jimenez R 

PROVIDER: S-EPMC3087858 | BioStudies | 2011-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2009-01-01 | S-EPMC2745927 | BioStudies
2021-01-01 | S-EPMC7921703 | BioStudies
2016-01-01 | S-EPMC4783373 | BioStudies
2018-01-01 | S-EPMC6316601 | BioStudies
1000-01-01 | S-EPMC4112637 | BioStudies
2018-01-01 | S-EPMC6316508 | BioStudies
2001-01-01 | S-EPMC58711 | BioStudies
2015-01-01 | S-EPMC4618214 | BioStudies
1000-01-01 | S-EPMC1283818 | BioStudies
2012-01-01 | S-EPMC3245471 | BioStudies