Unknown

Dataset Information

0

Different patterns of expansion, contraction and memory differentiation of HIV-1 Gag-specific CD8 T cells elicited by adenovirus type 5 and modified vaccinia Ankara vaccines.


ABSTRACT: The magnitude and functional quality of antiviral CD8 T cell responses are critical for the efficacy of T cell based vaccines. Here, we investigate the influence of two popular viral vectors, adenovirus type 5 (Ad5) and modified vaccinia Ankara (MVA), on expansion, contraction and memory differentiation of HIV-1 Gag insert-specific CD8 T cell responses following immunization and show different patterns for the two recombinant viral vectors. The Ad5 vector primed 6-fold higher levels of insert-specific CD8 effector T cells than the MVA vector. The Ad5-primed effector cells also underwent less contraction (<2-fold) than the MVA-primed cells (>5-fold). The Ad5-primed memory cells were predominantly CD62L negative (effector memory) whereas the MVA-primed memory cells were predominantly CD62L positive (central memory). Consistent with their memory phenotype, MVA-primed CD8 T cells underwent higher fold expansion than Ad5-primed CD8 T cells following a homologous or heterologous boost. Impressively, the Ad5 boost changed the quality of MVA-primed memory response such that they undergo less contraction with effector memory phenotype. However, the MVA boost did not influence the contraction and memory phenotype of Ad5-primed response. In conclusion, our results demonstrate that vaccine vector strongly influences the expansion, contraction and the functional quality of insert-specific CD8 T cell responses and have implications for vaccine development against infectious diseases.

SUBMITTER: Pillai VK 

PROVIDER: S-EPMC3139022 | BioStudies | 2011-01-01

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3592123 | BioStudies
2017-01-01 | S-EPMC5521799 | BioStudies
2012-01-01 | S-EPMC3458867 | BioStudies
2020-01-01 | S-EPMC7449457 | BioStudies
1000-01-01 | S-EPMC1887603 | BioStudies
2013-01-01 | S-EPMC3979587 | BioStudies
2014-01-01 | S-EPMC4071140 | BioStudies
2012-01-01 | S-EPMC3334902 | BioStudies
1000-01-01 | S-EPMC3528774 | BioStudies
2014-06-03 | E-GEOD-50764 | ArrayExpress