Unknown

Dataset Information

0

Genome-wide identification of in vivo binding sites of GlxR, a cyclic AMP receptor protein-type regulator in Corynebacterium glutamicum.


ABSTRACT: Corynebacterium glutamicum GlxR is a cyclic AMP (cAMP) receptor protein-type regulator. Although over 200 GlxR-binding sites in the C. glutamicum genome are predicted in silico, studies on the physiological function of GlxR have been hindered by the severe growth defects of a glxR mutant. This study identified the GlxR regulon by chromatin immunoprecipitation in conjunction with microarray (ChIP-chip) analyses. In total, 209 regions were detected as in vivo GlxR-binding sites. In vitro binding assays and promoter-reporter assays demonstrated that GlxR directly activates expression of genes for aerobic respiration, ATP synthesis, and glycolysis and that it is required for expression of genes for cell separation and mechanosensitive channels. GlxR also directly represses a citrate uptake gene in the presence of citrate. Moreover, ChIP-chip analyses showed that GlxR was still able to interact with its target sites in a mutant with a deletion of cyaB, the sole adenylate cyclase gene in the genome, even though binding affinity was markedly decreased. Thus, GlxR is physiologically functional at the relatively low cAMP levels in the cyaB mutant, allowing the cyaB mutant to grow much better than the glxR mutant.

SUBMITTER: Toyoda K 

PROVIDER: S-EPMC3147711 | BioStudies | 2011-01-01

REPOSITORIES: biostudies

Similar Datasets

2011-12-02 | E-GEOD-26870 | ArrayExpress
2011-12-02 | GSE26870 | GEO
2020-01-01 | S-EPMC7026483 | BioStudies
2004-01-01 | S-EPMC415749 | BioStudies
2014-01-01 | S-EPMC4254451 | BioStudies
1000-01-01 | S-EPMC3624568 | BioStudies
2020-01-09 | GSE140408 | GEO
2006-01-01 | S-EPMC1347311 | BioStudies
2012-01-01 | S-EPMC3406165 | BioStudies
| PRJEB36438 | ENA