Dataset Information


Endo-beta-1,3-galactanase from winter mushroom Flammulina velutipes.

ABSTRACT: Arabinogalactan proteins are proteoglycans found on the cell surface and in the cell walls of higher plants. The carbohydrate moieties of most arabinogalactan proteins are composed of ?-1,3-galactan main chains and ?-1,6-galactan side chains, to which other auxiliary sugars are attached. For the present study, an endo-?-1,3-galactanase, designated FvEn3GAL, was first purified and cloned from winter mushroom Flammulina velutipes. The enzyme specifically hydrolyzed ?-1,3-galactan, but did not act on ?-1,3-glucan, ?-1,3:1,4-glucan, xyloglucan, and agarose. It released various ?-1,3-galactooligosaccharides together with Gal from ?-1,3-galactohexaose in the early phase of the reaction, demonstrating that it acts on ?-1,3-galactan in an endo-fashion. Phylogenetic analysis revealed that FvEn3GAL is member of a novel subgroup distinct from known glycoside hydrolases such as endo-?-1,3-glucanase and endo-?-1,3:1,4-glucanase in glycoside hydrolase family 16. Point mutations replacing the putative catalytic Glu residues conserved for enzymes in this family with Asp abolished activity. These results indicate that FvEn3GAL is a highly specific glycoside hydrolase 16 endo-?-1,3-galactanase.


PROVIDER: S-EPMC3149374 | BioStudies | 2011-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-01-01 | S-EPMC4148793 | BioStudies
2006-01-01 | S-EPMC1472343 | BioStudies
2017-01-01 | S-EPMC5541203 | BioStudies
2009-01-01 | S-EPMC2802879 | BioStudies
2008-01-01 | S-EPMC2293163 | BioStudies
2018-01-01 | S-EPMC6217937 | BioStudies
1000-01-01 | S-EPMC4673573 | BioStudies
2017-01-01 | S-EPMC5686792 | BioStudies
1000-01-01 | S-EPMC168684 | BioStudies
2010-01-01 | S-EPMC2953027 | BioStudies