Dataset Information


Characterization of multidrug-resistant Escherichia coli isolates from animals presenting at a university veterinary hospital.

ABSTRACT: In this study, we examined molecular mechanisms associated with multidrug resistance (MDR) in a collection of Escherichia coli isolates recovered from hospitalized animals in Ireland. PCR and DNA sequencing were used to identify genes associated with resistance. Class 1 integrons were prevalent (94.6%) and contained gene cassettes recognized previously and implicated mainly in resistance to aminoglycosides, ?-lactams, and trimethoprim (aadA1, dfrA1-aadA1, dfrA17-aadA5, dfrA12-orfF-aadA2, bla(OXA-30)-aadA1, aacC1-orf1-orf2-aadA1, dfr7). Class 2 integrons (13.5%) contained the dfrA1-sat1-aadA1 gene array. The most frequently occurring phenotypes included resistance to ampicillin (97.3%), chloramphenicol (75.4%), florfenicol (40.5%), gentamicin (54%), neomycin (43.2%), streptomycin (97.3%), sulfonamide (98.6%), and tetracycline (100%). The associated resistance determinants detected included bla(TEM), cat, floR, aadB, aphA1, strA-strB, sul2, and tet(B), respectively. The bla(CTX-M-2) gene, encoding an extended-spectrum ?-lactamase (ES?L), and bla(CMY-2), encoding an AmpC-like enzyme, were identified in 8 and 18 isolates, respectively. The mobility of the resistance genes was demonstrated using conjugation assays with a representative selection of isolates. High-molecular-weight plasmids were found to be responsible for resistance to multiple antimicrobial compounds. The study demonstrated that animal-associated commensal E. coli isolates possess a diverse repertoire of transferable genetic determinants. Emergence of ES?Ls and AmpC-like enzymes is particularly significant. To our knowledge, the bla(CTX-M-2) gene has not previously been reported in Ireland.

PROVIDER: S-EPMC3194860 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC3477587 | BioStudies
| S-EPMC3194884 | BioStudies
| S-EPMC4597763 | BioStudies
| S-EPMC4700134 | BioStudies
| S-EPMC3663672 | BioStudies
| S-EPMC3347111 | BioStudies
2015-01-01 | S-EPMC4457809 | BioStudies
| S-EPMC4399151 | BioStudies
| S-EPMC3528966 | BioStudies
| S-EPMC6963716 | BioStudies