Unknown

Dataset Information

0

Pathogen-induced inflammatory environment controls effector and memory CD8+ T cell differentiation.


ABSTRACT: In response to infection, CD8(+) T cells integrate multiple signals and undergo an exponential increase in cell numbers. Simultaneously, a dynamic differentiation process occurs, resulting in the formation of short-lived effector cells (SLECs; CD127(low)KLRG1(high)) and memory precursor effector cells (CD127(high)KLRG1(low)) from an early effector cell that is CD127(low)KLRG1(low) in phenotype. CD8(+) T cell differentiation during vesicular stomatitis virus infection differed significantly than during Listeria monocytogenes infection with a substantial reduction in early effector cell differentiation into SLECs. SLEC generation was dependent on Ebi3 expression. Furthermore, SLEC differentiation during vesicular stomatitis virus infection was enhanced by administration of CpG-DNA, through an IL-12-dependent mechanism. Moreover, CpG-DNA treatment enhanced effector CD8(+) T cell functionality and memory subset distribution, but in an IL-12-independent manner. Population dynamics were dramatically different during secondary CD8(+) T cell responses, with a much greater accumulation of SLECs and the appearance of a significant number of CD127(high)KLRG1(high) memory cells, both of which were intrinsic to the memory CD8(+) T cell. These subsets persisted for several months but were less effective in recall than memory precursor effector cells. Thus, our data shed light on how varying the context of T cell priming alters downstream effector and memory CD8(+) T cell differentiation.

SUBMITTER: Obar JJ 

PROVIDER: S-EPMC3208080 | BioStudies | 2011-01-01

REPOSITORIES: biostudies

Similar Datasets

2007-01-01 | S-EPMC2034442 | BioStudies
1000-01-01 | S-EPMC5390367 | BioStudies
2012-03-01 | E-GEOD-36168 | ArrayExpress
2012-03-01 | GSE36168 | GEO
2013-01-01 | S-EPMC3711656 | BioStudies
2011-01-01 | S-EPMC3226770 | BioStudies
1000-01-01 | S-EPMC4507483 | BioStudies
1000-01-01 | S-EPMC4496536 | BioStudies
2011-01-01 | S-EPMC4222684 | BioStudies
1000-01-01 | S-EPMC2806751 | BioStudies