Dataset Information


Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins.

ABSTRACT: Chemoreceptor arrays are supramolecular transmembrane machines of unknown structure that allow bacteria to sense their surroundings and respond by chemotaxis. We have combined X-ray crystallography of purified proteins with electron cryotomography of native arrays inside cells to reveal the arrangement of the component transmembrane receptors, histidine kinases (CheA) and CheW coupling proteins. Trimers of receptor dimers lie at the vertices of a hexagonal lattice in a "two-facing-two" configuration surrounding a ring of alternating CheA regulatory domains (P5) and CheW couplers. Whereas the CheA kinase domains (P4) project downward below the ring, the CheA dimerization domains (P3) link neighboring rings to form an extended, stable array. This highly interconnected protein architecture underlies the remarkable sensitivity and cooperative nature of transmembrane signaling in bacterial chemotaxis.


PROVIDER: S-EPMC3309718 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2004-01-01 | S-EPMC536031 | BioStudies
2012-01-01 | S-EPMC3418435 | BioStudies
2012-01-01 | S-EPMC3418421 | BioStudies
2019-01-01 | S-EPMC6827319 | BioStudies
2018-01-01 | S-EPMC5866786 | BioStudies
1000-01-01 | S-EPMC1820660 | BioStudies
2010-01-01 | S-EPMC2873776 | BioStudies
2013-01-01 | S-EPMC3922707 | BioStudies
2018-01-01 | S-EPMC5915782 | BioStudies
2006-01-01 | S-EPMC2904553 | BioStudies