Unknown

Dataset Information

0

Propiconazole is a specific and accessible brassinosteroid (BR) biosynthesis inhibitor for Arabidopsis and maize.


ABSTRACT: Brassinosteroids (BRs) are steroidal hormones that play pivotal roles during plant development. In addition to the characterization of BR deficient mutants, specific BR biosynthesis inhibitors played an essential role in the elucidation of BR function in plants. However, high costs and limited availability of common BR biosynthetic inhibitors constrain their key advantage as a species-independent tool to investigate BR function. We studied propiconazole (Pcz) as an alternative to the BR inhibitor brassinazole (Brz). Arabidopsis seedlings treated with Pcz phenocopied BR biosynthetic mutants. The steady state mRNA levels of BR, but not gibberellic acid (GA), regulated genes increased proportional to the concentrations of Pcz. Moreover, root inhibition and Pcz-induced expression of BR biosynthetic genes were rescued by 24epi-brassinolide, but not by GA(3) co-applications. Maize seedlings treated with Pcz showed impaired mesocotyl, coleoptile, and true leaf elongation. Interestingly, the genetic background strongly impacted the tissue specific sensitivity towards Pcz. Based on these findings we conclude that Pcz is a potent and specific inhibitor of BR biosynthesis and an alternative to Brz. The reduced cost and increased availability of Pcz, compared to Brz, opens new possibilities to study BR function in larger crop species.

SUBMITTER: Hartwig T 

PROVIDER: S-EPMC3348881 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5217202 | BioStudies
2016-01-01 | S-EPMC5398191 | BioStudies
2019-01-01 | S-EPMC6775476 | BioStudies
2017-01-01 | S-EPMC5665367 | BioStudies
2020-01-01 | S-EPMC7409705 | BioStudies
1000-01-01 | S-EPMC4581192 | BioStudies
2019-01-01 | S-EPMC6892407 | BioStudies
2020-01-01 | S-EPMC7411146 | BioStudies
2008-01-01 | S-EPMC2423651 | BioStudies
2011-01-01 | S-EPMC3166115 | BioStudies