Unknown

Dataset Information

0

Actin cytoskeleton controls movement of intracellular organelles in pancreatic duct epithelial cells.


ABSTRACT: In most eukaryotic cells, microtubules and filamentous actin (F-actin) provide tracks on which intracellular organelles move using molecular motors. Here we report that cytoplasmic movement of both mitochondria and lysosomes is slowed by F-actin meshwork formation in pancreatic duct epithelial cells (PDEC). Mitochondria and lysosomes were labeled with fluorescent Mitotracker Red CMXRos and Lysotracker Red DND-99, respectively, and their movements were monitored using epi-fluorescence and confocal microscopy. Mitochondria and lysosomes moving actively at rest stopped rapidly within several seconds after an intracellular Ca(2+) rise induced by activation of P2Y(2) purinergic receptors. The 'freezing' of the organelles was inhibited by blocking the Ca(2+) rise or by pretreatment with latrunculin B, an inhibitor of F-actin formation. Indeed, this freezing effect on the organelles was accompanied by the formation of F-actin in the whole cytoplasm as stained with Alexa 488-phalloidin in fixed PDEC. For real-time monitoring of F-actin formation in live cells, we expressed sGFP-fimbrin actin binding domain2 (fABD2) in PDEC. Rapid recruitment of the fluorescent probe near the nucleus and lysosomes suggested dense F-actin formation around intracellular structures. The development of F-actin paralleled that of organelle freezing. We conclude that rapid Ca(2+)-dependent F-actin formation physically restrains intracellular organelles and reduces their mobility non-selectively in PDEC.

SUBMITTER: Jung SR 

PROVIDER: S-EPMC3390015 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2003-01-01 | S-EPMC206996 | BioStudies
2019-01-01 | S-EPMC6293086 | BioStudies
2009-01-01 | S-EPMC3049446 | BioStudies
2013-01-01 | S-EPMC4086488 | BioStudies
2018-01-01 | S-EPMC6209448 | BioStudies
2017-01-01 | S-EPMC5681906 | BioStudies
1998-01-01 | S-EPMC1219772 | BioStudies
2013-01-01 | S-EPMC3603520 | BioStudies
1000-01-01 | S-EPMC4499241 | BioStudies
2011-01-01 | S-EPMC3173716 | BioStudies