Unknown

Dataset Information

0

Neutrophil chemotaxis within a competing gradient of chemoattractants.


ABSTRACT: The dynamics of neutrophil chemotaxis under competing chemoattractant gradients was studied using a microfluidic platform. This microfluidic platform, which establishes a stable and dynamic gradient of chemoattractants across a cell culture chamber, enabled the investigation of human neutrophil migration patterns in the presences of four different chemoattractants (leukotriene B(4), chemokine C-X-C motif ligands 2 and 8, and fMLP) and competing gradients of all pairwise combinations. The migration patterns for individual cells were tracked and quantitatively analyzed, and the results suggest a hierarchy among these chemoattractants of fMLP > CXCL8 > CXCL2 > leukotriene B(4). In all conditions, over 60% of neutrophils exposed to a competing gradient move toward the stronger signal though the weaker chemoattractant still influences neutrophil motility. These results yield insight about how each chemoattractant contributes to overall neutrophil chemotaxis within complex physiological environments.

SUBMITTER: Kim D 

PROVIDER: S-EPMC3404751 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-01-01 | S-EPMC3897492 | BioStudies
2019-01-01 | S-EPMC6422936 | BioStudies
2013-01-01 | S-EPMC3867262 | BioStudies
2013-01-01 | S-EPMC3867270 | BioStudies
1000-01-01 | S-EPMC5687044 | BioStudies
1000-01-01 | S-EPMC1940227 | BioStudies
2018-01-01 | S-EPMC6176921 | BioStudies
2012-01-01 | S-EPMC4141281 | BioStudies
2017-01-01 | S-EPMC5511520 | BioStudies
2020-01-01 | S-EPMC7851090 | BioStudies