Dataset Information


Effective elicitation of human effector CD8+ T Cells in HLA-B*51:01 transgenic humanized mice after infection with HIV-1.

ABSTRACT: Humanized mice are expected to be useful as small animal models for in vivo studies on the pathogenesis of infectious diseases. However, it is well known that human CD8(+) T cells cannot differentiate into effector cells in immunodeficient mice transplanted with only human CD34(+) hematopoietic stem cells (HSCs), because human T cells are not educated by HLA in the mouse thymus. We here established HLA-B*51:01 transgenic humanized mice by transplanting human CD34(+) HSCs into HLA-B*51:01 transgenic NOD/SCID/Jak3(-/-) mice (hNOK/B51Tg mice) and investigated whether human effector CD8(+) T cells would be elicited in the mice or in those infected with HIV-1 NL4-3. There were no differences in the frequency of late effector memory and effector subsets (CD27(low)CD28(-)CD45RA(+/-)CCR7(-) and CD27(-)CD28(-)CD45RA(+/-)CCR7(-), respectively) among human CD8(+) T cells and in that of human CD8(+) T cells expressing CX3CR1 and/or CXCR1 between hNOK/B51Tg and hNOK mice. In contrast, the frequency of late effector memory and effector CD8(+) T cell subsets and of those expressing CX3CR1 and/or CXCR1 was significantly higher in HIV-1-infected hNOK/B51Tg mice than in uninfected ones, whereas there was no difference in that of these subsets between HIV-1-infected and uninfected hNOK mice. These results suggest that hNOK/B51Tg mice had CD8(+) T cells that were capable of differentiating into effector T cells after viral antigen stimulation and had a greater ability to elicit effector CD8(+) T cells than hNOK ones.


PROVIDER: S-EPMC3412802 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-10-24 | E-GEOD-54867 | BioStudies
2014-01-01 | S-EPMC3900423 | BioStudies
2010-01-01 | S-EPMC2948507 | BioStudies
2019-01-01 | S-EPMC6436856 | BioStudies
1000-01-01 | S-EPMC5596574 | BioStudies
2011-01-27 | E-GEOD-26890 | ArrayExpress
2011-01-27 | GSE26890 | GEO
1000-01-01 | S-EPMC4773556 | BioStudies
2015-01-01 | S-EPMC4403403 | BioStudies
2017-01-01 | S-EPMC5421808 | BioStudies